Tuesday, February 18, 2014

Online tools for CRISPR target identification and screening

There are 2 main ones I've used:

Zhang lab (MIT) CRISPR design site
For identification of standard 23-base targets, scored by a metric that minimizes likelihood of off-target cleavage.  Also for locating paired targets for paired-nickase approach.  

From the Zinc Finger Consortium; originally for zinc finger & TALEN target screening, adapted for CRISPR .  Allows identification of shorter targets for the truncated-target approach.